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Chemical standards were prepared in ultrapure water and were analysed under identical conditions as samples to validate metabolite identities by chromatographic retention time and MS/MS fragmentation pattern matching

The slides were mounted with a solution containing DAPI (Molecular Probes) and imaged using a Leica TCS SP8 laser scanning confocal microscope (Leica Microsystems CMS GmbH, Wetzlar, Germany)

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These results showed that TNF- inhibited EBV reactivation in EBV-associated tumour cells, and the inhibitory effect of TNF- on EBV reactivation was lost after inhibition of the TNFR1 receptor