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However, clinical research increasingly recognized that patients with metabolic diseases frequently experience clusters of systemic complications, including heart failure (HF), chronic kidney disease (CKD), and fatty liver disease, that single-target drugs historically failed to address comprehensively

Additionally, metabolic improvements may reduce fatigue, mood changes, and the insulin-driven carbohydrate cravings that plague many PCOS patients

Samples were run in duplicates, and 16S DNA gene expression was calculated as a relative ratio of Ct value of target normalized with Ct value of genomic DNA