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For extraction of proteins on cell membrane cell cytoplasm, cell samples were pretreated using a membrane and cytosol protein extraction kit (Beyotime Biotechnology) according to the protocol from manufacture

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Long-term complications from poorly controlled type 2 diabetes include heart disease, stroke, diabetic retinopathy (and subsequent blindness), kidney failure, and diminished peripheral blood flow which may lead to amputations

Grading of evidence At the outcome level, the GRADE guidelines were utilised to evaluate the quality of evidence across all included studies

Following a second wash at identical conditions, gels were collected, weighed and resuspended in a sufficient volume of 500 mM NaCl buffer to ensure a final salt concentration of 170 mM