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For immunofluorescent staining, tissue sections were incubated with anti-insulin antibody (Abcam), followed by a secondary fluorophore-conjugated antibodies (Invitrogen), or with FITC conjugated anti-mouse CD11c (BD Bioscience), or with Cy5 conjugated anti-mouse F4/80 (eBioscience) and counterstained with 4,6-diamidino-2-phenylindole (DAPI) (Sigma)

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How will I know if my patient has been approved

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10.1016/j.ijsu.2015.04.033 91

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