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Furthermore, another study has demonstrated (Wei et al., 2022) Fc reduces tubular injury and alleviates mitochondrial dysfunction in AKI mice, in part through the modulation of the SIRT3/SOD2 pathway

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The extracted RNA was reverse transcribed into cDNA with HiScript III RT SuperMix for qPCR (R323-01, Vazyme, China), and cDNA was used to perform qRTPCR with the ChamQ Universal SYBR qPCR Master Mix (Q711-02, Vazyme, China)