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Fluorescent secondary antibodies included doneky anti-rabbit Alexa 405, donkey anti-rabbit Alexa 488, doneky anti-rabbit Alexa 594, donkey anti-mouse Alexa 594, donkey anti-chicken 647, donkey anti-gunieapig Alexa 594, and donkey anti-gunieapig Alexa 647 were sourced from Jackson ImmunoResearch Laboratories and diluted (1:500) in the blocking solution

These data are consistent with the T allele decreasing risk for T2D, as well as decreasing glucose and HbA1c levels, but slightly increasing the risk of smoking initiation (Supplementary Table 16)

This scheduling allows researchers to manage multiple experimental phases or coordinate with institutional protocols

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