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Cytocompatibility assay The cytocompatibility of DNZ-NLPs against SH-SY5Y cells was determined using the MTT assay

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190 Paracrine FGF21 in the liver can increase liver regeneration and antioxidant capacity and inhibit liver aging 191 and metabolic dysfunction-associated steatohepatitis

Excretion Pathways Limited data on combined excretion indicates: Primarily renal elimination of peptide fragments Hepatic metabolism contributes to overall clearance Copper recycling through normal physiological pathways No evidence of accumulation in chronic dosing studies (animal models) The relationship between rapid plasma clearance and prolonged biological effects represents a key area requiring further mechanistic investigation across all three components