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Whilst the dynamic range of this assay was low in our hands, making it challenging to apply to higher throughput screening efforts or to concentration responses, it could be adapted by the use of alternative fluorophores with greater signal separation from the nanoluciferase emission peak (Dale et al ., 2019) or using complementation approaches (Inoue et al ., 2019)

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Ask any orthopedic surgeon what makes tendons and ligaments so frustrating to treat, and you'll get some version of the same answer: they barely have a blood supply