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Phase II sticks a water-soluble tag onto that handle
N Engl J Med 1981
McClain T, et al
In Vitro Methods Cell Culture At-T20 mouse pituitary tumor cells were obtained from ATCC (CCL-89) and cultured in high-glucose Dulbeccos minimal essential medium (DMEM) complete media [10% fetal calf serum + penicillin and streptomycin (Penn/Strep)]
In both *in vitro* models using hepatocyte cell lines (e.g., HepG2) and *in vivo* rodent models fed high-fat or methionine-choline deficient (MCD) diets, this formulation is used to investigate the mechanisms of hepatic steatosis
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