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To preclude reoxygenation, all cells designated for Western blot analysis or RNA extraction were subjected to three sequential washes with PBS within the hypoxic workstation, followed immediately by lysis using the appropriate buffer (RIPA lysis or Trizol reagent)

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Effects of serotonin agonists and doxapram on respiratory depression and hypoxemia in etorphine-immobilized impala ( Aepyceros melampus )

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