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For apical to basolateral (AB) and basolateral to apical (BA) permeability, compounds were added at 10 M concentration to the apical (A) side and basolateral (B) side, respectively, and the corresponding amount of permeation was determined by measuring compound concentration on the B or A side

It used a ShapiroWilk test to assess normality and an F-test to assess homoscedasticity, and attempted log-transformation to resolve any lack thereof 40