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A 1 mL aliquot was removed from the release medium and replaced with an equivalent volume of fresh PBS at 0, 1, 2, 4, 8, 12, 24, 48, and 72 h to maintain sink conditions

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doi: 10.4049/jimmunol.167.4.2060 186 RobinsonGAPengJPeckhamHButlerGPineda-TorraICiurtinCet al

Routes of Administration Subcutaneous injection (standard): Protocols describe injection in the abdomen, thigh, or upper arm, rotating sites between injections, using standard SC technique with a 29-31 gauge insulin syringe