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The researchers evaluated various oxidative stress markers, including protein carbonylation, protein-bound 4-hydroxy-2-nonenal (HNE), levels of total and oxidized glutathione (GSH), concentrations of heat shock protein (HSP) and thioredoxin (TRX), in amniotic fluid samples

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RPMI 1640 medium without l-glutamine, l-cysteine, l-cystine and l-methionine was used as the base medium for different medium conditions (MP Biomedicals, 1646454)