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In a state of vitamin B 12 deficiency, the cell responds by redirecting folate metabolic pathways to supply increasing amounts of methyltetrahydrofolate

For lentiviral particles production, the full-length cDNA-encoding mito GCL, or its mutant inactive E103A form, was first digested with Eco 47III /Sma I to generate blunt ends, and then subcloned in the Pme I site of pWPI lentiviral vector (Didier Trono laboratory, Lausanne, Switzerland)

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doi:10.1016/j.ctim.2019.102234 McFarlin BK, Henning AL, Bowman EM, Gary MA, Carbajal KM