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For Ki67 and p-ERK stainings, blocking and primary antibody dilution were performed using PB buffer composed of 0.05% milk powder (#T145.2, Carl Roth, Germany), 0.25% fish gelatin (#G7765, Sigma-Aldrich, USA), 0.5% Triton X-100 (#BP151-100, Fisher Bioreagents, Germany), 20 mM HEPES (pH 7.2, #9105.4, Carl Roth, Germany), and 0.9% NaCl (#3957.1, Carl Roth, Germany)