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To ensure the absorbance from oil red O staining was within the linear detection range of the plate reader, a calibration curve was established for oil red O staining in adipocytes using a previously described protocol.[23] 2.12

Patients should return unused vials to the pharmacy or follow local disposal protocols

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Of course, this depends upon the cause of your deficiency such as malabsorption, pernicious anemia and other conditions that create vitamin B12 deficiencies and if the cause has been effectively treated

Your healthcare teams goal will be to continue them until its safe to stop (if possible)