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The effect of high-mobility group box 1 protein on activity of regulatory T cells after thermal injury in rats

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sgRNA (CAGCCAAAATCACAGAATGA) targeting N-terminus of SNIP1 gene was cloned into pX330-BbsI-PITCh (addgene #127875) 121 and microhomology arms surrounding CRISPR-Cas9 cleavage site (each 20 bp long) were introduced into donor plasmid pCRIS-PITChv2-Puro-dTAG (addgene #91793) 71