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The main cortisol secretory phase runs during sleep hours 6 through 8

At the end of each treatment, 4 mg/kg carprofen (Norocarp vet, 50 mg / mL, N-Vet AB, Uppsala, Sweden) was administered SC for 24 h of post procedural analgesia

The HPLC gradient grade acetonitrile (ACN), used for chromatographic analysis, hydrochloric acid utilized for the standard solution preparation, sodium hydrogen phosphate heptahydrate (Na 2 HPO 4 7H 2 O), sodium dihydrogen phosphate dihydrate (NaH 2 PO 4 2H 2 O) and sodium hydroxide were purchased from J.T

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Statements Author contributions CL and GK participated in the design, writing, and final corrections of the manuscript