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HGH is a hormone secreted by the pituitary gland

Western blotting Small intestinal tissue lysates were obtained by tissue homogenization in lysis buffer (10 mM TrisHCl pH 7.6, 150 mM NaCl, 5 mM EDTA, 1 mM PMSF, 1 mM DTT, 0.25% Nonidet P-40, and protease inhibitor)

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