A whole avocado with a meal significantly increases GLP-1 and peptide YY levels while reducing subsequent hunger
The synthesis method of the acylated GLP-1 compound and modified gene thereof is characterized by comprising the following steps of preparing a compound 2, namely, taking GLP-1 (9-37) as an initial raw material, carrying out PEG medication by a compound 1 on Lys of a position 26 of a relative sequence GLP-1 (9-37), controlling the reaction pH and carrying out an acylation coupling reaction to obtain a compound 2
RT-PCR was done using a standard SYBR-green PCR kit (SYBR Premix EX TaqTM II, Takara), and the gene-specific PCR amplification was conducted using the Applied Biosystems StepOnePlus Real-Time PCR System (Applied Biosystems, USA)
Then, the supernatant was transferred to the Falcon tubes and centrifuged at 20,000 g for one and a half hours
Zepbound has the same active ingredient as Mounjaro (tirzepatide), but is approved by the FDA only for weight loss