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Finally, after being washed three times in IP buffer, protein blot analysis was conducted on the immunocomplexes bound to the anti-Strep II-Tag mAb or rabbit anti-DDDDK-tag (Flag) mAb

doi:10.1371/journal.pone.0272299

Based on the established mechanisms by which acupuncture modulates programmed necrosis in intracerebral hemorrhage models, combined with the known roles of programmed cell death (PCD) in PD pathogenesis and the demonstrated ability of acupuncture to regulate multiple PCD forms (e.g., autophagy, apoptosis) in PD models, we hypothesize that acupuncture may also inhibit the assembly and execution of the core necroptotic pathway

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