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incubation (37C) + 60 min

Semaglutide is a pure GLP-1 receptor agonist, meaning it targets only the GLP-1 pathway

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The difficulty was that testing for low blood glucose necessitated laboratory equipment and technical skills that were then not available to most physicians

Total RNA was extracted from snap frozen platypus tissues (frontal cortex, pancreas, liver, lung, small intestine, stomach, heart, venom gland, testis, muscle, lymph and kidney) and echidna tissue (small intestine, pancreas, liver, venom gland, heart and brain) using TRIzol (Invitrogen, USA) according to the manufactures instructions