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Avoid harmful lifestyle factors like smoking and excessive alcohol consumption Treatment Options For those struggling with poor egg quality, several treatment options are available: - Medications to enhance egg quality - Advanced reproductive techniques such as IVF with ICSI (Intracytoplasmic Sperm Injection) - Preimplantation Genetic Testing (PGT) to select chromosomally normal embryos - In some cases, using donor eggs may be recommended It's important to consult with a fertility specialist to determine the best course of action based on individual circumstances

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2.6 Indirect immunofluorescence For cell culture, monolayers of cells were grown in 48-well plates, grouped and treated after the cells were rinsed three times with PBS, and fixed with 4% formaldehyde for 15 min, followed by three washes with PBS

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