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The main methods include knocking out essential genes, making engineered bacteria dependent on exogenous nutrients, or setting up suicide switches

[21] Within cells, cyanocobalamin is converted to methylcobalamin and 5- deoxyadenosylcobalamin, coenzymes that participate respectively in homocysteine remethylation to methionine and in the isomerization of methylmalonyl-CoA to succinyl CoA, reactions essential for DNA synthesis, erythropoiesis, and myelin maintenance

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The cultured medium was then removed, and 100 L of DMSO was added