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2.2 Mice and treatment Eight-week-old male C57BL/6J mice sourced from Shanghai Slack Experimental Animals Co., Ltd (License number SCXK 2022-0004) were randomly allocated into four cohorts (n = 5/group)

doi: 10.3389/fonc.2022.898971 16 YamadaNKatoHKawaguchiMSuzuiNMiyazakiTNaganoAet al

Psychiatric disturbances as a first clinical symptom of Wilsons disease case report

Cells were maintained in DMEM (HCT116, SW480, U2OS), MEM- with 1 ng ml 1 fibroblast growth factor 2 (Peprotech, hMSC) or DMEM with 1% non-essential amino acid (Gibco) and 50 g ml 1 uridine (Sigma-Aldrich) supplementation (143B), with 10% dialysed foetal bovine serum (dFBS), 50 U ml 1 penicillin and streptomycin (ThermoFisher, 15070063) and 1 mM sodium pyruvate (ThermoFisher, 11360070)

These data demonstrate MT-II as an effective agent for improving autism-like behavioral deficits in the adult male MIA mouse model of autism