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The RT-qPCR design was replicated with biological replicates for each treatment condition, treating each plate as a statistical block 128
Subsequently, Western Blot (WB) analysis detected a decrease in total P53 expression but an increase in phosphorylated P53 (p-P53) expression in OGD-induced senescent endothelial cells post FOXO4-DRI treatment (Figures 5B,C)
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doi: 10.1530/eje.0.1320012 21 BonertVCarmichaelJWuZMirochaJPerezDAClarkeNJet al
They are commonly combined in research planning because they act on two different GH pathways, and GHRH-plus-secretagogue pairings have amplified GH pulses in human secretagogue studies
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