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IPEC-J2 cell line culture Porcine intestinal epithelial cell line-J2 (IPEC-J2) was cultured in DMEM/F12 supplemented with 10% fetal bovine serum, 100 UI/mL penicillin-streptomycin, and 1x ITSX (Gibco, Carlsbad, CA, USA) under 37 humidified environment with 5% CO 2

While this approach addresses important aspects of aging, it does not directly target cellular repair mechanisms