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A major limitation of WCBs is that bacterial cultures must be maintained and continuously cultured over multiple generations under antibiotic selection, which could result in the accumulation of mutations that ultimately disrupt WCB performance

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Historically, transcription factors like C/EBP have posed therapeutic challenges due to their absence of enzymatic activity and well-defined small-molecule binding sites, rendering them difficult to target directly with traditional drugs (Hattori et al., 2003)

It is a key gene in the methylation cycle