According to the Melbourne Consensus, recently published in Nature Reviews: Gastroenterology & Hepatology, excess pancreatic fat accumulation is now formally classified as a disease state associated with increased risk of type 2 diabetes, pancreatitis, and pancreatic cancer independent of alcohol consumption
Remember that sustainable weight management is a journey, not a destination
Adipose tissue was minced into 3-4mm pieces and transferred to 50 ml conical tubes containing 10 ml of digestion buffer [Kreb-Ringers-HEPES buffer pH 7.4 (10 mM HEPES, 2.2 mM CaCl2, 120 mM NaCl, 1.2 mM KH2PO4, 1.2 mM MgSO4, 4.7 mM KCl) + 1 mg/ml type VIII collagenase (C2139, Sigma)], mixed by inversion and incubated at 37 C for 1h in a shaking water bath at 150 rpm
Le Burel S, Champiat S, Mateus C, et al
The purpose of the data review was to confirm the absence of extreme, potentially unsafe reductions in insulin doses, carbohydrate intake or weight, and the absence of unsafe glucose levels (for example, time spent below 3.9 mmol l 1 more than 4%) in either intervention
