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A.VaqueroC.LoiraI.CuerdaR.PalomeroF.et al

Replicate experiments across multiple batches can help verify consistency and robustness

CastejnGrin M, Herraiz C, Olivares C, JimnezCervantes C, GarcaBorrn JC

Endotoxin (LAL) Limulus Amebocyte Lysate assay ensures endotoxin levels are within safe limits

2-Acetamidophenol upregulates the expression of glutathione synthesis-related genes (such as GCLC, GCLM, GSS) and iron ion transport genes (such as FPN1, FTH), reduces the accumulation of intracellular reactive oxygen species (ROS) and ferrous ions (Fe 2+ ), and enhances the activity of glutathione peroxidase GPX4 , thereby inhibiting macrophage phagocytosis of oxidized low-density lipoprotein (ox-LDL) and foam cell formation