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[49] demonstrated that an overexpression of RACK1 enhanced the activity of IGF-1R kinase, whereas, the IGF-1R-induced activation of PI3K/AKT signaling was inhibited in mouse fibroblasts and MCF-7 cells

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The column temperature was 30 C, and the mobile phase was a mixture of A [50 mM phosphate buffer (pH 3.0) containing 10 mM sodium 1-heptanesulfonate] and B (methanol) with an A/B ratio of 95/5 at a flow rate of 1 mL/min