The following day, cells were transfected with 20 nM of siRNA targeting CD36 (sc-29995) from Santa Cruz Biotechnology (Santa Cruz Biotechnology, Dallas, Texas, USA) using Lipofectamine RNAiMAX Reagent from Thermo Fisher Scientific in Opti-MEM media from Gibco accordingly to manufacturers instructions for 48 h, refreshed every day before being treated or not with PA (200 M) during 24 h
Jeremias A, Soodini G, Gelfand E, Xu Y, Stanton RC, Horton ES, et al
doi: 10.1016/j.fertnstert.2004.10.010 Chiara De Luca, Elena V
Reduces Inflammation Helps combat oxidative stress, which can contribute to aging and chronic conditions
However, no significant effect was observed on the rates of heart failure or myocardial reinfarction (DiNicolantonio et al