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To analyze postprandial incretin secretion, we fasted PAR2-mutant and WT mice overnight followed by refeeding of normal chow diet (NCD) for 1 hour, a reliable time point as determined by a pilot experiment in WT mice on both C57BL/6N and C57BL/6J backgrounds (Supplemental Figure 1B)

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Enhanced GLP-1 Activity & Longevity: Increases GLP1R expression: The system boosts the expression of GLP1R , the gene that produces GLP-1 receptors