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Primary cells are another option which have much higher TEER and lower permeability than the available cell lines, but they are more time consuming, expensive, and can be passaged a limited number of times

Because glutamine is the source of -KG, glutaminase inhibitors such as BPTES interfere with -KG homeostasis and lead to inhibited proliferation in cancer cells carrying this mutant IDH1 (Seltzer et al., 2010)

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