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However, whereas eNAP-1 is present only in very low quantities in equine neutrophils, eNAP-2 seems to be one of the major gene products [32]

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Proteins were precipitated using 150 l water, 50 l chloroform and 200 l methanol, with intense vortex followed by centrifugation (21,000 g , 10 minutes at 4 C)

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