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Amplification specificity was confirmed by melting curve analysis and agarose gel electrophoresis
1:1000) for 1 h at room temperature in the dark
Due to shunting inhibition, this value is more depolarized (-55 mV) than the mean value or resting value of membrane potential 154
Tissue lysates were then rotated for 15 min at 4 C and centrifuged at 12,000 g for 15 min at 4 C
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