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Criticisms of studies purporting to show a gateway effect from e-cigarette use to smoking among adolescents include inadequate adjustment for potential confounders [5] and lack of negative controls [65]

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miRNA in serum exosomes markedly regulated the expression of AMPK and CYP7A1 In our experiment, a miRNA-291a-3p expression level was shown to be significantly decreased by RTPCR, while miRNA-126a-5p was increased (Fig