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1 g/mL ionomycin and 100 ng/mL phorbol 12-myristate 13-acetate were added to the culture media with 0.01% Protein Transport Inhibitor (BD GolgiStopTM, BD biosciences, NJ, USA)

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I open the test with shaking fingers and hover over the seat, trying to hold the flimsy cardboard stick steady between my legs