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Functional profiling Prior to functional gene prediction using PICRUSt (phylogenetic investigation of communities by reconstruction of unobserved states) described by Statistical analysis The community diversity was assessed using the number of OTUs, Shannon diversity index ( H ), Chao 1, and Simpson evenness index

Pulmonary function tests Pulmonary function tests or spirometry can measure the airflow into and out of the lungs and be used to predict the severity of emphysema

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