Liquid chromatography and mass spectrometry conditions Analyses of urinary nicotine, cotinine and NNAL were conducted using an HPLC (UltiMate 3000, Thermo Fisher Scientific, Yokohama, Japan) coupled to a hybrid quadrupole-Orbitrap mass spectrometer (Thermo Scientific Q Exactive Focus) with heated electrospray ionization (HESI-II)
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is to examine the smoking question by drawing upon evidence that is widely scattered through the biological sciences