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The aim is to sniff as much tabocco as possible from a tobacco package of five grammes within a time limit of one minute
387f(d) to include the higher minimum age of retail sale for any tobacco product, including ecigarettes (because of the FDA deeming action described in the next section)
A bipolar stimulating electrode (PlasticOne 2-channels, stainless-steel, 10 mm) for ICSS was then implanted unilaterally (left or right, randomized) in the brain (stereotaxic coordinates from bregma according to mouse after Paxinos atlas: AP 1.4 mm, ML 1.2 mm, DV 4.8 mm from the brain)
Nicotine is a naturally occurring alkaloid found in the tobacco plant
Several EU Member States, such as Belgium, Estonia, Germany, and Slovakia, have banned the use of animals for the development and testing of tobacco products
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