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Tobacco was a labor-intensive crop and ambitious planters, with seemingly limitless land before them, lacked only laborers to exponentially escalate their wealth and status

Cultivated tobacco seeds from roughly 3,500 years ago have been found at archaeological sites in the southern United States, and evidence of the plants domestication in South America stretches back almost 8,000 years

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Further, the 3 terminal sequence was confirmed by 3 RACE using FirstChoice RLM RACE kit (Thermo Fischer Scientific, USA)