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Mitochondrial oxygen consumption rate measurement BEAS-2B cells (CSE exposed and unexposed) were trypsinized and collected in RPMI-1640 cell culture medium, 100 l/well of the cell suspension at cell density 50,000 cells/well was seeded in duplicates in a Seahorse XF Pro96 microplate (Agilent Technologies, Santa Clara, CA, USA)

Prior Presentations Portions of these data were presented previously in abstract form at the American Thoracic Society 2015 International Conference (AJRCCM 2015;191:A3908)

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The test has gone through several iterations since its introduction, but it remains in use to this day, and is still one of the main ways of assessing addiction

doi:10.1177/096228029800700202