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Human primary blood-derived monocytes (pBDMCs) were isolated from donor buffy coats using the EasySep Direct Human Monocyte Isolation Kit (Stemcell, #19669), which isolates primary CD14 + monocytes via negative immunomagnetic selection according to the manufacturers protocol (Supplementary Fig

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Other approaches include reducing tobacco consumption, by use of either pharmacotherapies, behavioral strategies, or polices that restrict access to tobacco products