Primary antibodies against HIF-1 (610958, BD Biosciences, Franklin Lakes, NJ), hydroxy-HIF-1 (3434, Cell Signaling Technology, Danvers, MA), HIF-2 (NB100-122, Novus Biologicals, Littleton, CO), TOM20 (11802-1-AP, Proteintech Group, Rosemont, IL), HSP60 (15282-1-AP, Proteintech Group), and Tubulin (sc-8035, Santa Cruz Biotechnology, Dallas, TX) were diluted in blocking buffer and incubated overnight at 4 C
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Generally in plant cell models, cytosolic Ca 2+ is known to be a modulator of PCD induced during various occasions (both positive and negative way) such as abiotic stress responses [9, 10] and hypersensitive defense response against pathogen attacks [19, 20]
