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We propose to substitute the monomethoxy PEG monoacid used during this synthesis by PEG monoacid having a reactive function blocked at the end of the chain as the commercial product: Boc-PEG-carbonateNHS, MW 5000, Boc = tert- butoxycarbonylBoc-PEG-carbonate NHS, MW 5000, Boc = tert-butoxycarbonyl Rfrence SUNBRIGHT BO-050TS, NOF Corporation Aprs raction, comme indiqu dans l'exemple, sauf que nous utiliserons des mlanges MeO-PEG-COOH et Boc-PEG-carbonateNHS (rapports massiques 1 :0.05 1 : 0,5) la place de MeO-PEG-COOH, la deprotection se fera par rajout d'acide trifluoroactique (TFA) Protocole possible :Reference SUNBRIGHT BO-050TS, NOF Corporation After reaction, as shown in the example, except that we will use mixtures MeO-PEG-COOH and Boc-PEG-carbonateNHS (mass ratios of 1: 0.05 to 1: 0.5) to the place of MeO-PEG-COOH, the protection will be by addition of trifluoroacetic acid (TFA) Possible protocol: On rajoute 0.6 mL de TFA une suspension de0.6 mL of TFA is added to a suspension of 300 mg de nanoparticules dans 2 mL d'eau
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