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The virus silencing vector pTRV2-GFP- NtTBL31 was constructed by double enzyme digestion method ( Nco I and Mlu I), and the recombinant plasmid was transformed into ( Agrobacterium tumefaciens ) GV3101 strain

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PacBio Iso-seq RNA-seq library was also constructed using total RNA from the leaf sample, which was sequenced on a PacBio Sequel II platform

All ECs contain a small heating element that vaporizes a refill liquid to generate the aerosol