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After washing for 10 min at room temperature in 0.1 M Na cacodylate buffer PBS, sections were fixed in 2.5% glutaraldehyde in 0.1 M Na cacodylate buffer (pH7.3) overnight at 4 C, washed again for 10 min 0.1 M Na cacodylate buffer and post-fixed in 1% OsO 4 in 0.1 M Na cacodylate buffer for 1 h

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