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Osteoclast formation and bone resorption assay Osteoclast formation assays were performed by culturing 1 10 5 /well non-adherent bone marrow cells in 96-well plates in 0.1 ml of -MEM with 10% FBS for 7 days

Exposure to ETS at work Jamrozik (2005) gives the prevalence of passive smoking at work as 11%, an estimate that probably derives from the survey commissioned by ASH in April 1999, which revealed that approximately 3 million people in the UK are regularly exposed to ETS at work (ASH, 2004)

Nicotine strongly activates dendritic cellmediated adaptive immunity: potential role for progression of atherosclerotic lesions