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In another study, tobacco-dependent smokers who are carriers of null or inactive alleles (CYP2A6*2 or CYP2A6*3) were observed to smoke fewer cigarettes per week than smokers with two active CYP2A6 alleles (Pianezza et al., 1998)

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Cytotoxicity was determined by the by release of lactate dehydrogenase (LDH) using the CytoTox 96 non-reactive cytotoxicity as per manufacturers instructions (Promega)

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This is due to repeated and rapid contractions increasing metabolic demand in the muscles, swiftly depleting energy stores, and accumulating lactate, a metabolic byproduct contributing to the sensation of fatigue 17